Inheritance and identification of ISSR-RGA markers associated with powdery mildew resistance in mungbean for marker-assisted breeding

Mungbean (Vigna radiata (L.) R. Wilczek var. radiata) yield is dramatically constrained by powdery mildew (PM) caused Sphaerotheca phaseoli (Z.Y. Zhao) U. Braun (1985), which considerably prevalent in the cool-dry season of production South, East, and Southeast Asia countries including Thailand. Exploitation varieties resistant to disease crucial meet sustainable production. A population 64 F2:9 F2:10 recombinant inbred lines (RILs) generated hybridization susceptible parent ‘Chai Nat 72’ (CN72) with ‘V4758’ was used assess genetic resistance identify inter simple sequence repeat-anchored gene analog (ISSR-RGA) markers linked PM gene. The response these RILs visually scored field during winter seasons, twice 2015 2018, segregation pattern determined chi-square test (χ2). resulting ratios 1:1 indicated a qualitative nature dominantly inherited conferring resistance. When bulk segregant analysis (BSA) undertaken using 378 ISSR-RGA primer combinations among both parents DNA bulks individuals, 11 exhibited polymorphisms, one marker I41tP379 closest revealed highly significant correlation (R2 (%) = 26; P < 0.001) logarithm odd (LOD) score 5.85. could trace molecular assisted breeding for mungbean improvement.